SUBDIVIDE AND CONQUER RESOLUTION

This contribution will be freewheeling in the domain of signal, image and surface processing and touch briefly upon some topics that have been close to the heart of people in our research group. A lot of the research of the last 20 years in this domain that has been carried out world wide is dealing with multiresolution. Multiresolution allows to represent a function (in the broadest sense) at different levels of detail. This was not only applied in signals and images but also when solving all kinds of complex numerical problems. Since wavelets came into play in the 1980's, this idea was applied and generalized by many researchers. Therefore we use this as the central idea throughout this text. Wavelets, subdivision and hierarchical bases are the appropriate tools to obtain these multiresolution effects. We shall introduce some of the concepts in a rather informal way and show that the same concepts will work in one, two and three dimensions. The applications in the three cases are however quite different, and thus one wants to achieve very different goals when dealing with signals, images or surfaces. Because completeness in our treatment is impossible, we have chosen to describe two case studies after introducing some concepts in signal processing. These case studies are still the subject of current research. The first one attempts to solve a problem in image processing: how to approximate an edge in an image efficiently by subdivision. The method is based on normal offsets. The second case is the use of Powell-Sabin splines to give a smooth multiresolution representation of a surface. In this context we also illustrate the general method of construction of a spline wavelet basis using a lifting scheme

The RNA modification database, RNAMDB: 2011 update

Since its inception in 1994, The RNA Modification Database (RNAMDB, http://rna-mdb.cas.albany.edu/RNAmods/) has served as a focal point for information pertaining to naturally occurring RNA modifications. In its current state, the database employs an easy-to-use, searchable interface for obtaining detailed data on the 109 currently known RNA modifications. Each entry provides the chemical structure, common name and symbol, elemental composition and mass, CA registry numbers and index name, phylogenetic source, type of RNA species in which it is found, and references to the first reported structure determination and synthesis. Though newly transferred in its entirety to The RNA Institute, the RNAMDB continues to grow with two notable additions, agmatidine and 8-methyladenosine, appended in the last year. The RNA Modification Database is staying up-to-date with significant improvements being prepared for inclusion within the next year and the following year. The expanded future role of The RNA Modification Database will be to serve as a primary information portal for researchers across the entire spectrum of RNA-related research

A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity, high stability and low toxicity

The use of chemically synthesized short interfering RNAs (siRNAs) is currently the method of choice to manipulate gene expression in mammalian cell culture, yet improvements of siRNA design is expectably required for successful application in vivo. Several studies have aimed at improving siRNA performance through the introduction of chemical modifications but a direct comparison of these results is difficult. We have directly compared the effect of 21 types of chemical modifications on siRNA activity and toxicity in a total of 2160 siRNA duplexes. We demonstrate that siRNA activity is primarily enhanced by favouring the incorporation of the intended antisense strand during RNA-induced silencing complex (RISC) loading by modulation of siRNA thermodynamic asymmetry and engineering of siRNA 3′-overhangs. Collectively, our results provide unique insights into the tolerance for chemical modifications and provide a simple guide to successful chemical modification of siRNAs with improved activity, stability and low toxicity

Field-testing of a rapid survey method to assess the prevalence and causes of hearing loss in Gao'an, Jiangxi province, China.

BACKGROUND: The Rapid Assessment of Hearing Loss (RAHL) survey protocol aims to measure the prevalence and causes of hearing loss in a low cost and rapid manner, to inform planning of ear and hearing services. This paper reports on the first field-test of the RAHL in Gao'an County, Jiangxi Province, China. This study aimed to 1) To report on the feasibility of RAHL; 2) report on the estimated prevalence and causes of hearing loss in Gao'an. METHODS: A cross-sectional population-based survey was conducted in September-October 2018. Forty-seven clusters in Gao'an County were selected using probability-proportionate-to-size sampling. Within clusters, compact segment sampling was conducted to select 30 people aged 50+. A questionnaire was completed covering sociodemographics, hearing health, and risk factors. Automated pure-tone audiometry was completed for all participants, using smartphone-based audiometry (hearTest), at 0.5, 1, 2, 4 kHz (kHz). All participants had their ears examined by an Ear Nose and Throat (ENT) doctor, using otoscopy, and probable causes of hearing loss assigned. Prevalence estimates were age and sex standardised to the Jiangxi population. Feasibility of a cluster size of 30 was examined by assessing the response rate, and the proportion of clusters completed in 1 day. RESULTS: 1344 of 1421 eligible participants completed the survey (94.6%). 100% of clusters were completed in 1 day. The survey was completed in 4.5 weeks. The prevalence of moderate or greater hearing loss (pure-tone average of 0.5, 1, 2, 4 kHz of > = 41dBHL in the better ear) was 16.3% (95% CI = 14.3, 18.5) and for any level of hearing loss (pure-tone average of > = 26dBHL in the better ear) the prevalence was 53.2% (95% CI = 49.2, 57.1). The majority of hearing loss was due to acquired sensorineural causes (91.7% left; 92.1% right). Overall 54.0% of the population aged 50+ (108,000 people) are in need of diagnostic audiology services, 3.4% were in need of wax removal (7000 people), and 4.8% were in need of surgical services (9500 people). Hearing aid coverage was 0.4%. CONCLUSION: The RAHL survey protocol is feasible, demonstrated through the number of people examined per day, and the high response rate. The survey was completed in a much shorter period than previous all-age surveys in China. Some remaining challenges included assignment of causes of probable sensorineural loss. The data obtained from this survey can be used to scale-up hearing services in Gao'an

Linear viscoelastic behavior of copper phthalocyanine dispersions used in printing inks

In this paper we describe the linear viscoelastic properties of copper phthalocyanine (CuPCN) dispersions that are used in the manufacturing of offset lithographic printing inks. Transmission electron microscopy shows that the primary pigment particles are rod-like and have sizes in the range of 10 to 300 nm. Steady shear measurements show that the dispersions are Newtonian at a pigment volume fraction of 0.073 and become increasingly shear thinning as the pigment volume fraction is increased. The strong shear-thinning nature of these dispersions can be attributed to the highly flocculated nature of the dispersions, which is due to interparticle attractions. The structural complexity of the dispersions also results in an unexpected linear viscoelastic response. While at low frequencies (0.1 and 1.0 Hz) the ex tent of the linear region decreases with increasing pigment concentration, at a higher frequency (10 Hz) the extent of the linear region increases with increasing pigment concentration. This increase in the linear region with increasing pigment concentration suggests that at higher frequencies the dispersion is less brittle, and that the rheological behavior is dominated by intra-aggregate associations. In addition, frequency sweeps show that the dispersions behave like a viscoelastic liquid at low pigment concentrations. However, at higher pigment concentrations (yet significantly lower than the maximum packing fraction) the dispersions behave like a cross-linking polymer at its gel point.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/47209/1/397_2004_Article_BF00403533.pd

Synthesis of 2-amino-6-acetamidomethyl-9-(beta-d-ribofuranosyl) purine

status: publishe

A nonlinear contour preserving transform for geometrical image compression

info:eu-repo/semantics/publishe

Adaptive splitting for stabilizing 1-D wavelet decompositions

info:eu-repo/semantics/publishe

Normal Offsets for digital image compression

info:eu-repo/semantics/publishe

Adaptive splitting for stabilizing 1-D wavelet decompositions on irregular grids

This paper proposes a scheme to improve the stability of wavelet decompositions on 1-D irregular grids. Wavelet transforms on irregular grids are constructed using the lifting scheme. The filters in this scheme take the structure of the grid into account. Nice as it is, however, we undoubtedly bump into numerical stability issues directly related to the irregularity of the grid. Existing stabilizing methods concentrate on the filters used in the lifting scheme itself. While this may be effective in reducing the instability, they are inadequate when a highly irregular grid is involved. The approach presented in this paper is different, as it concentrates on the subsampling or subdivision. Grid locations in the multiscale transform are inserted in such an order that the irregularity of the grid at coarse scales is kept under control. This way, the proposed algorithm prevents instability at coarse scales, rather than healing it. Simulations illustrate that the proposed multiscale decomposition scheme is much more stable than the currently available transforms, especially at coarse scales, where effects of instability have a wide range